Objective: To Phone case observe the protective effect of Nigella sativa on number of cystic follicles in Letrozole induced polycystic ovaries in mice.Study Design: Laboratory based randomized control trial.Place and Duration of Study: Department of Anatomy, Army Medical College in collaboration with National Institute of Health from Nov 2014 to Nov 2015.Material and Methods: Forty female BALB/c mice were selected and divided in four groups, each having 10 animals.
Group A served as control and was given normal diet.Group B was given Letrozole at a dose of 1milligram/kilogram body weight.Group C was treated with Letrozole for eight weeks at a dose of 1milligram/kilogram body weight and Nigella sativa seeds powder at a dose of 10grams/kilogram body weight once daily starting at 22 day and continued up to eight weeks.Group D was treated with Letrozole for eight weeks at a dose of 1milligram/kilogram body weight and Nigella sativa oil at a dose of 4milliliter/kilogram body weight once daily Ladders starting at 22 day and continued up to eight weeks.
Animals were dissected a day after last dose.Size, shape, color and consistency of ovary was observed.Right ovary was processed, embedded and stained for histological study.Number of cystic follicles were counted and noted.
Results: Significant number of cystic follicles was observed in ovaries of animals of group B as compared to group A.While their number decreased significantly in group C and D as compared to group B.Conclusion: Nigella sativa seeds powder and its oil, both have a similar protective effect on histomorphology of ovary of polycystic ovarian syndrome (PCOS) in mice by decreasing the number of cystic follicles.